Plasmid

Part:BBa_K4808009:Design

Designed by: Zhao Guichun   Group: iGEM23_AIS-China   (2023-10-10)


pTarget


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 330
    Illegal XbaI site found at 336
    Illegal SpeI site found at 221
    Illegal PstI site found at 348
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 330
    Illegal NheI site found at 198
    Illegal SpeI site found at 221
    Illegal PstI site found at 348
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 330
    Illegal BglII site found at 360
    Illegal BamHI site found at 186
    Illegal XhoI site found at 384
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 330
    Illegal XbaI site found at 336
    Illegal SpeI site found at 221
    Illegal PstI site found at 348
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 330
    Illegal XbaI site found at 336
    Illegal SpeI site found at 221
    Illegal PstI site found at 348
    Illegal NgoMIV site found at 1155
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 122


Design Notes

none


Source

Li Q, Sun B, Chen J, Zhang Y, Jiang Y, Yang S. A modified pCas/pTargetF system for CRISPR-Cas9-assisted genome editing in Escherichia coli. Acta Biochim Biophys Sin (Shanghai). 2021;53(5):620-627. doi:10.1093/abbs/gmab036

References